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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been noticed in many tumors, which leads to chromosome instability, and so may possibly contribute to tumorigenesis. This protein was found to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have already been explained. Two added splice variants had been claimed but thorough nucleotide sequence information will not be however out there.
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Blocking peptides are peptides that bind particularly for the goal antibody and block antibody binding. These peptide commonly consists of the epitope acknowledged because of the antibody.
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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This manual outlines additional attainable optimization of cellular and immunoassay parameters to be certain the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind to the epitope about the focus on protein. By evaluating the staining within the blocked antibody vs . the antibody by itself, you can see which staining is precise.
Target Information and facts Cyclin E belongs on the highly conserved cyclin spouse and children, whose customers are characterized by a dramatic periodicity in protein abundance in the cell cycle. Cyclins function as regulators of CDK kinases. check here Unique cyclins show distinct expression and degradation styles which lead on the temporal coordination of each mitotic party. Cyclin E sorts a fancy with and capabilities being a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is particularly degraded as cells development by means of S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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